DNAzyme-based probe for circulating microRNA detection in peripheral blood

DNAzyme-based probe for circulating microRNA detection in peripheral blood

BACKGROUND The recent discovery of microRNAs (miRNAs) and their extracellular presence suggest a potential role of these regulatory molecules in defining the metastatic potential of cancer cells and mediating the cancer-host communication. This study aims to improve the sensitivity of miRNA detection via DNAzyme-based method and enhance the selectivity by using the DNAzyme-based probe to reduce...

Detection of microRNA Expression in Human Peripheral Blood Microvesicles

BACKGROUND MicroRNAs (miRNA) are small non-coding RNAs that regulate translation of mRNA and protein. Loss or enhanced expression of miRNAs is associated with several diseases, including cancer. However, the identification of circulating miRNA in healthy donors is not well characterized. Microvesicles, also known as exosomes or microparticles, circulate in the peripheral blood and can stimulate...

Circulating microRNA profiles for detection of peripheral arterial disease: small new biomarkers for cardiovascular disease.

Negative Regulation of Semaphorin-3A Expression in Peripheral Blood Mononuclear Cells Using MicroRNA-497-5p

Background: Semaphorin-3A (Sema3A), as a secreted semaphorin, is an immune modulator molecule participating in the pathogenesis of autoimmune diseases. MicroRNAs (miRNAs) modulate the target-gene expression at the post-transcriptional level. It has been proposed that miRNAs may be crucial to the modulation of the function of semaphorins. Previous findings have proven that miR-497-5p is upregula...

Simple, colorimetric detection of microRNA based on target amplification and DNAzyme.

Considering the crucial role played by microRNAs (miRNAs) in biological processes, we developed a novel strategy for simple and colorimetric detection of miRNA by combining target amplification with DNAzyme. Throughout the work, a 22-nt oligonucleotide sequence was used as a model analyte. A label-free hairpin probe (HP) was used as a simple platform for sensing the target. In the presence of t...